The Seibold Laboratory is a cutting-edge, NIH funded, laboratory focused on elucidating the pathobiological basis of asthma and other complex lung and allergic diseases. Our goal is to discover pathobiological subgroups of disease (termed disease endotypes) and the genetic, environmental, and immune factors driving their development. We are accomplishing these goals through the integration of genomic endotyping studies of large patient cohorts with in vitro mechanistic studies of human organoid culture models. Ultimately, we will leverage the information gained from our research to develop precision medicine approaches for the diagnosis, management, and treatment of these diseases. The Seibold Laboratory is composed of a multi-disciplinary team of "wet lab" cell, airway, and molecular biologists integrated with "dry lab" computational biologists, bioinformaticians, and statistical geneticists.
To learn more about the lab, please visit https://www.seibold-lab.com.
We are seeking a highly motivated candidate with a background in Stem or Lung Cell Biology, Molecular Genetics, and/or Genetics for a post-doctoral, Research Associate - Genetics position in the Seibold Lab. The research in our laboratory focuses on translational genetic and genomic studies of complex lung diseases. This position will be focused on determining the molecular function of genetic disease variants in airway epithelial cells using primary cell culture systems. The successful candidate will use cutting edge molecular tools including CRISPR’s to edit the genome of primary airway and iPS cells to study the function of genetic disease variants. This candidate will also lead studies investigating the molecular effects of environmental exposures on airway epithelial cells and the differentiation of airway stem cells into mucociliary epithelia. The successful applicant will be responsible for performing experiments in the laboratory, which will include tissue culture, cloning, cell exposures and infections, flow cytometry, transfections, real-time PCR, Nex-gen sequencing, fluorescent microscopy, and various cellular assays. The successful applicant will be expected to learn and develop techniques as required by the research project. The successful applicant will be expected to independently lead assigned research projects, which will include experimental design and data analysis, as well as manuscript and presentation preparation.
Successful candidates in the Postdoctoral Research Associate - Genetics position will have:
- Ph.D. in Stem Cell Biology, Cell Biology, Molecular Genetics, Genetics, Immunology, or a related field is required.
- Excellent written and verbal communication
- Effective judgment and problem solving with little supervision
- Highly motivated and committed to biomedical research
- Multi-tasking and a willingness to learn new technologies
- Cell culturing skills
- Molecular biology skills
Preferred skills and experience:
- Primary airway epithelial culture
- iPSC derivation and differentiation
- Work with Genome-editing technologies
- Gene expression work including Q-PCR or Nex-gen sequencing
- Microscopy skills
- Flow Cytometry
For questions please contact Dr. Max A. Seibold, email@example.com
To perform, on a very independent basis, all phases of experimental genetics/genomics investigations and to collaborate with Principal Investigators and senior researchers in the progress of major genetics/genomics research projects. To act as technical advisor to laboratory technical, support and auxiliary staff.
- Performs, on a very independent basis, all phases of experimental genetics/genomics investigations, to include being responsible to the Principal Investigator in the planning, developing, operating, recording, collaborating and reporting of major genetics/genomics projects.
- Collaborates with Principal Investigators and senior researchers in the progress of major research projects.
- Provides guidance to laboratory staff in one or more laboratory areas, including technicians, support and auxiliary staff. Performs as the technical advisor on complex analyses of gene and genetic variant function through the performance and use gene-editing technologies and 2D, 3D, organoid cell culture models, and other sophisticated techniques.
- Serves as primary contact with researcher staff for technical services on major genetic/genomic techniques, recording devices and statistical procedures relative to the specifically assigned field.
- Reviews quality control of new or modified laboratory techniques which affect any part of major projects.
- Performs routine and non-routine tests leading to the successful completion of developed experiments. Knows and follows age-specific guidelines.
- Performs as a back-up to staff absences and vacancies, as necessary.
- Prepares scientific manuscripts related to the genetic/genomic analyses conducted.
- Performs all other duties as assigned.
- Performs, as directed, safety compliance and uses Personal Protective Equipment (PPE), as needed.
- Participates in Quality Assessment (QA) and Quality Improvement (QI) programs, as directed.
- Ensures compliance with The Joint Commission and all other Federal, State and Regulatory Agencies.
- Responds promptly and sincerely to customer’s needs, requests and concerns via all communication forms using easily understood language and refraining from using inappropriate language and non-verbal gestures.
- Maintains positive working relationships as a team player through problem solving issues, speaking positively about others, listening attentively and observing the Patient Bill of Rights and Confidentiality.
- Increases customer service knowledge, skill and ability by participating in department and institution-wide specific programs.
- Incorporates National Jewish’s identity (Breathing Science is Life®) into daily functions. Speaks positively about the institution, provides customers with prompt service, maintains a clean and safe working environment, dresses appropriately based upon National Jewish safety standards, and departmental policies and wears an ID badge visibly.
- Education: M. D.; or, Ph. D. in genetics, genomics, biology, chemistry or other basic sciences required.
- Work Experience: Experience acquired in earning a Ph. D. in a specified basic science field.
- Special Training, Certification and Licensure: Extensive knowledge of standard research practices and methods in the conduct of biomedical research, but specifically in the conduct of gene-editing and complex 2D, 3D, and organoid tissue culture systems. Ability to design and plan complex genetic and molecular biology experiments. Ability to determine the approach to a research problem which will give the most significant results and to develop, lay out and plan the actual experiments to be performed, and ability to devise new methods and techniques as needed in experimentation. Ability to plan, organize and direct work of supporting personnel. Ability to prepare and write reports on research projects. Ability to establish and maintain effective working relationships with associates, other research personnel and the general public.